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  • All fields: samples
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Display: 20

    • Page 402

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    • City Manager Anderson reported on the properties that may be available for an additional 9 holes for the Golf Course. Nov. 17, 1 9 6 6 . . . A letter was read from Governor Rampton about home fallout protection. Survey will be conducted to...
    • Page 29

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    • 23 of the school, McLaughlin noticed that other Western-based institutions, such as the local Christian churches, provided religious reading material in Navajo and that Navajo literacy classes were established by members of the community. In terms...
    • Page 9

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    • Employee Giving 10 money over to administrators to use how they see fit. Darla also provided me with confidence regarding the timing of our campaign. She pointed out that the only reason BYU does a fall campaign is that the United Way of Utah...
    • Page 8

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    • 8 consists of images or profiles from unsolved cases. CODIS is an index that aids crime labs to store and compare DNA profiles. This record is called a CODIS DNA profile and consists of an individual’s DNA profile, along with the sample’s...
    • Page 10

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    • 10 Applied Biosystems (AB 2006, Foster City, CA) and the PowerPlex 16 produced by Promega Corporation (Promega 2008, Madison, WI.). The kit used in this study was the Promega PowerPlex16 multiplex kit, which detects sixteen STRs while the Applied...
    • Page 11

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    • 11 common in evidence due to degradation or even contamination, such as from other DNA or inhibitors. The more loci that can be detected allow for much more discrimination between individuals and thus simpler for identification among those...
    • Page 13

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    • 13 double-stranded DNA, some are specific for single-stranded molecules, others are active towards both and still others can cleave residues at the ends of DNA molecules or even anywhere along the DNA chain thus providing damage to the DNA....
    • Page 14

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    • 14 number of glass microscope slides were touched by a donor and held for 3-5 seconds. This was to ensure that a solid print was placed on each slide and therefore a sufficient source for the DNA to be collected. The experiment had two trials, with...
    • Page 15

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    • 15 shown not to interfere with the DNA extraction and amplification from evidence ( Balogh et al., 2003; Grubwieser, 2003; Roux et al., 1999.) 2.2 DNA Extraction DNA recovered from the latent print was extracted from epithelial cells following...
    • Page 16

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    • 16 Samples were quantified with the real-time PCR machine Roche Lightcycler 480 (Roche Applied Science, Mannhelm, Germany) using the AB Quantifiler Human DNA Quantification kit (Applied Biosystems, Foster City, California). This step was performed...
    • Page 17

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    • 17 concentrations of samples were then obtained and volumes were determined for use with PCR machine. 2.5 AMPLIFICATION Protocol used for PCR amplification was from all sections of the Promega PowerPlex 16 system technical manual (TM 012 Promega...
    • Page 18

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    • 18 of the ladders was completed prior to the sample run, and the peak heights were too high, the loading cocktail was modified to contain 0.25μl of ILS 600 and 9.75μl of formamide. Due to limit of chemical resources, only those samples that had...
    • Page 19

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    • 19 appearance of the latent fingerprint also increased (Figure 3). Figure 3 depicts physical fingerprint area data for weeks 1-3 only; this is because at the beginning of the experiment no degradation had taken place at the initial start of those...
    • Page 20

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    • 20 temperature treatment and incubation times with physical fingerprint area and DNA concentration. For the % physical degradation within the two trials over the temperature ranges and incubation times, percents varied between ± 0.5-1.5...
    • Page 22

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    • 22 Table 1: Percent of samples with their % degradation for that particular temperature range. Temperature % degradation 25°C 50°C 75°C 0.0 -2.0 44% 28% 11% 3.0-5.0 17% 39% 11% 6.0-10.0 39% 33% 11% 11.0-20.0 0% 0% 66% Figure 3: Mean % physical...
    • Page 23

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    • 23 DNA Concentration (ng/μl)-2024681012255075Temperature (°C)Mean Concentration (ng/μl)Week 1Week 2Week 3Week 4(°C)(°C)(°C) Figure 4: Mean DNA concentrations in ng/μl obtained from physical fingerprint samples over temperature ranges....
    • Page 26

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    • 26 Number of Samples with Partial Profiles01234512345678910111213141516Number of STRs PresentNumber of Samples Figure 7: Samples that had partial profiles obtained. Out of a possible 16 STRs to be present in each sample: one sample had 2 STRs...
    • Page 27

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    • 27 4 DISCUSSION & CONCLUSIONS 4.1 FINGERPRINT In this study it was hypothesized that increased temperature would have a visual effect on the physical appearance of latent fingerprint. When looking at the print itself, it was observed that as...

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