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    • Page 25

    • "KH, ]G, TW,Volunteer Initials; RW, Right Wrist; MTH,Mouth; pas, Positive epithelial cell control; HB, Human Blood on Duct tape (positive Control); NEG,Negative control; lJNKN, Unknown epithelial cell sample ~T,NoTarget; OC,Overconcentrated; BT,...
    • Page 14

    • 10 Students understand the tremendous energy released in nuclear reactions is a result of small amounts of matter being converted to energy. P3.p1 Conservation of Energy (prerequisite) When energy is transferred from one system to another, the...
    • Page 15

    • 11 C3.2x Enthalpy Chemical reactions involve breaking bonds in reactants (endothermic) and forming new bonds in the products (exothermic). The enthalpy change for a chemical reaction will depend on the relative strengths of the bonds in the...
    • Page 16

    • 11 Table 1: Sample Identification. Surface trench depth and dry weight of samples before extraction. Sample Dry Weight (g) Depth (cm) Sample Dry Weight (g) Depth...
    • Page 17

    • 12 and reverse primer, 1X (5 μl) SYBR Green master mix and sterile distilled water to a final volume of 10μl (Roche, 2009). qPCR cycling conditions were 5 minutes at 95ºC followed by 45 cycles of 10 seconds at 95ºC, 10 seconds at 50ºC, and12...
    • Page 16

    • 12 Chemical interactions either release energy to the environment (exothermic) or absorb energy from the environment (endothermic). C3.4A Use the terms endothermic and exothermic correctly to describe chemical reactions in the laboratory. C3.4B...
    • Page 14

    • 12 However, “…even the most proactive teacher must follow through with a negative consequence for an inappropriate behavior. Yet, the worst time to select a punisher is during an episode of student misbehavior” (Akin-Little & Little, 2008, p....
    • Page 16

    • 16 Samples were quantified with the real-time PCR machine Roche Lightcycler 480 (Roche Applied Science, Mannhelm, Germany) using the AB Quantifiler Human DNA Quantification kit (Applied Biosystems, Foster City, California). This step was performed...
    • Page 17

    • 17 concentrations of samples were then obtained and volumes were determined for use with PCR machine. 2.5 AMPLIFICATION Protocol used for PCR amplification was from all sections of the Promega PowerPlex 16 system technical manual (TM 012 Promega...
    • Page 23

    • 19 concept addresses conservation of energy in chemical interactions. It does not address the concept of increase in disorder. This is a concept missing from the Utah Core and will be discussed later in the literature review. This standard is...
    • Page 6

    • 2 behind academically they lose a certain amount of self-confidence. This is especially crucial when dealing with math. The time restraints in a regular education classroom make it difficult for a teacher to address the varying problems of each...
    • Page 15

    • 2.5 QUANTIFICATION Following the extraction procedure, the samples were quantified using a Roche 480 Lightcycler real-time PCR machine, Indianapolis, IN, with a Plexor HY quantification kit from Promega Corporation, Madison WI [7]. Prior to using...
    • Page 7

    • 3 Illicit Methamphetamine and impurities Methamphetamine production in home laboratories using ephedrine or pseudoephedrine, the active ingredients in several over-the-counter drugs such as Claritin and Sudafed, lead to FDA restriction through the...
    • Page 31

    • 31 5 REFERENCES Applied Biosystems (2006). Quantifiler Human DNA Quantification Kit User’s Manual. Applied Biosystems Foster City, California. Chapters 1-6. Ayaly-Torres S , Chen Y, Svoboda T, Rosenblatt J and Van Houten B. Analysis of Gene...
    • Page 32

    • 32 Gunn, Alan. Essential Forensic Biology. Forensic DNA Typing. Wiley & Sons, Ltd. 2006. pp39-53 Kanable, Rebecca. DNA from fingerprints. Retrieved July 2005 Knowles A M. Aspects of physicochemical methods...
    • Page 40

    • 36 *Elements, Compounds, and Chemical Reactions A student will: a. Differentiate elements and compounds. b. Define chemical change. c. Compare and contrast substances and mixtures. d. Explain the reasoning behind the use of symbols for elements. e....
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    • 38 f. Apply the rules for writing the formulas of chemical compounds by using a flowchart, as measured by written assignments at 75% accuracy. **The Mole A student will: a. Describe methods of measuring the amount of matter. b. Define Avogadro’s...
    • Page 43

    • 39 c. Distinguish between a solvent and a solute. d. Describe what happens in the solution process. e. Explain why all ionic compounds are electrolytes. f. Distinguish between a suspension and a solution, as measured by written assignments, and a...
    • Page 8

    • 4 Table 2: Impurities found in amphetamine synthesized with the Leuckart reaction (Verweij, A.M., 1989). Name Source Formamide a Formic Acid a Phenylacetone a N-formylamphetamine b 4-Methyl-5-phenylpyrimidine c,d 4-Benzylpyrimidine c,d Dibenzyl...
    • Page 3

    • 4.2 Amplification/STR Analysis ........................................................................ Pages 28-29 Chapter 5 :...


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