Old folks party, many of these people lived in the Old Fort and moved to Cedar City in 1857, they are our real pioneers. First row: Uriah Goodfellow, John Chatterly, Timothy Adams, Evan Williams, John Adams, John Fretwell, Winnie Roche, ? Root, ?...
B. B. Roll Call
Shorty, Our High Mogul
Cullen, the Figurehead
Snookums, the Numskull
Smiley Tuck .
Samples were quantified with the real-time PCR machine Roche Lightcycler 480 (Roche Applied Science, Mannhelm, Germany) using the AB Quantifiler Human DNA Quantification kit (Applied Biosystems, Foster City, California). This step was performed...
Gunn, Alan. Essential Forensic Biology. Forensic DNA Typing. Wiley & Sons, Ltd. 2006. pp39-53
Kanable, Rebecca. DNA from fingerprints. Retrieved July 2005 http://www.officer.com/publication/article
Knowles A M. Aspects of physicochemical methods...
and reverse primer, 1X (5 μl) SYBR Green master mix and sterile distilled water to a final volume of 10μl (Roche, 2009). qPCR cycling conditions were 5 minutes at 95ºC followed by 45 cycles of 10 seconds at 95ºC, 10 seconds at 50ºC, and12...
2.2 EXTRACTION METHOD 1
For this method, a small cutting approximately 5 cm2 was taken from each sample of duct tape and placed into a separate Eppendorf tube. A phenol chloroform organic extraction protocol was then used to remove the DNA from the...
Following the extraction procedure, the samples were quantified using a Roche 480
Lightcycler real-time PCR machine, Indianapolis, IN, with a Plexor HY quantification kit from
Promega Corporation, Madison WI . Prior to using...