Old folks party, many of these people lived in the Old Fort and moved to Cedar City in 1857, they are our real pioneers. First row: Uriah Goodfellow, John Chatterly, Timothy Adams, Evan Williams, John Adams, John Fretwell, Winnie Roche, ? Root, ?...
B. B. Roll Call
Shorty, Our High Mogul
Cullen, the Figurehead
Snookums, the Numskull
Whitey Lumen
Fussy
Pott .
Black Paddy
Pinkie
Muggins
Smiley Tuck .
Tiny
Cupid
e.
James Esplin
Clarence Riddle
Willie Roche
Ervin Riddle
Loren Shurtz
Alfred...
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Samples were quantified with the real-time PCR machine Roche Lightcycler 480 (Roche Applied Science, Mannhelm, Germany) using the AB Quantifiler Human DNA Quantification kit (Applied Biosystems, Foster City, California). This step was performed...
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Gunn, Alan. Essential Forensic Biology. Forensic DNA Typing. Wiley & Sons, Ltd. 2006. pp39-53
Kanable, Rebecca. DNA from fingerprints. Retrieved July 2005 http://www.officer.com/publication/article
Knowles A M. Aspects of physicochemical methods...
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and reverse primer, 1X (5 μl) SYBR Green master mix and sterile distilled water to a final volume of 10μl (Roche, 2009). qPCR cycling conditions were 5 minutes at 95ºC followed by 45 cycles of 10 seconds at 95ºC, 10 seconds at 50ºC, and12...
2.2 EXTRACTION METHOD 1
For this method, a small cutting approximately 5 cm2 was taken from each sample of duct tape and placed into a separate Eppendorf tube. A phenol chloroform organic extraction protocol was then used to remove the DNA from the...
2.5 QUANTIFICATION
Following the extraction procedure, the samples were quantified using a Roche 480
Lightcycler real-time PCR machine, Indianapolis, IN, with a Plexor HY quantification kit from
Promega Corporation, Madison WI [7]. Prior to using...