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    • People, old folks party

    • People, old folks party
    • Cedar City (Iron County, Utah)--People
    • Old folks party, many of these people lived in the Old Fort and moved to Cedar City in 1857, they are our real pioneers. First row: Uriah Goodfellow, John Chatterly, Timothy Adams, Evan Williams, John Adams, John Fretwell, Winnie Roche, ? Root, ?...
    • 1915 77

    • 1915 77
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    • B. B. Roll Call Shorty, Our High Mogul Cullen, the Figurehead Snookums, the Numskull Whitey Lumen Fussy Pott . Black Paddy Pinkie Muggins Smiley Tuck . Tiny Cupid e. James Esplin Clarence Riddle Willie Roche Ervin Riddle Loren Shurtz Alfred...
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    • Page 16
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    • 16 Samples were quantified with the real-time PCR machine Roche Lightcycler 480 (Roche Applied Science, Mannhelm, Germany) using the AB Quantifiler Human DNA Quantification kit (Applied Biosystems, Foster City, California). This step was performed...
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    • Page 32
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    • 32 Gunn, Alan. Essential Forensic Biology. Forensic DNA Typing. Wiley & Sons, Ltd. 2006. pp39-53 Kanable, Rebecca. DNA from fingerprints. Retrieved July 2005 http://www.officer.com/publication/article Knowles A M. Aspects of physicochemical methods...
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    • Page 16
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    • 11 Table 1: Sample Identification. Surface trench depth and dry weight of samples before extraction. Sample Dry Weight (g) Depth (cm) Sample Dry Weight (g) Depth...
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    • Page 17
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    • 12 and reverse primer, 1X (5 μl) SYBR Green master mix and sterile distilled water to a final volume of 10μl (Roche, 2009). qPCR cycling conditions were 5 minutes at 95ºC followed by 45 cycles of 10 seconds at 95ºC, 10 seconds at 50ºC, and12...
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    • Page 31
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    • 26 Millward, H., Samowitz, W., Wittwer, C.T., & Bernard, P.S. 2002. Homogeneous amplification and mutation scanning of the p53 gene using fluorescent melting curves. Clin. Chem. 48:1321-1328, National Science Foundation. 2009. Biosciences:...
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    • Page 13
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    • 2.2 EXTRACTION METHOD 1 For this method, a small cutting approximately 5 cm2 was taken from each sample of duct tape and placed into a separate Eppendorf tube. A phenol chloroform organic extraction protocol was then used to remove the DNA from the...
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    • Page 15
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    • 2.5 QUANTIFICATION Following the extraction procedure, the samples were quantified using a Roche 480 Lightcycler real-time PCR machine, Indianapolis, IN, with a Plexor HY quantification kit from Promega Corporation, Madison WI [7]. Prior to using...
    • Page 37

    • Page 37
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    • REFERENCES 1. Hobbs, Andrea L; Gauntt, Jennifer; Keagy, Roger; Lowe, Preston C; Ward, Dennis. (2007) A New Approach for the Analysis ofDuct Tape Backings: Forensic Science Communications, 9(1). 2. Berg, Jim; Nyberg, Tim. Duct Tape 101:...

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