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    • TABLE OF CONTENTS Chapter 1 : Introduction & Background ........................................................................... Page 3 1.1 Duct...
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    • CHAPTER 1: INTRODUCTION & BACKGROUND Because duct tape is so versatile and readily available, it has become a commonly submitted piece of evidence. Duct tape is frequently used in cases involving homicides, sexual assaults, kidnappings, and...
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    • 1.1 DUCT TAPE Adhesive tape, more specifically, masking tape was invented sometime in the 1920's by Richard Drew. He worked for the Minnesota Mining and Manufacturing Company, now known as 3M. Duct tape was originally manufactured by the Johnson &...
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    • components) are added to one or more elastomers to provide stickiness. Finally, inorganic materials give the adhesive more volume, or color. Common additives include: calcite, dolomite, iron oxide, kaolinite, talc, titanium dioxide (rutile or...
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    • Chelex extraction methods are simpler and much faster than organic extraction. Chelex is an ion-exchange resin which means that it is made up of ions that act as chelating groups and bind to metal ions like magnesium. This removes magnesium from...
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    • conducted to determine if DNA could be collected at all from the duct tape. A duct tape sample with epithelial cells was run through a basic organic extraction protocol and following extraction the Plexor HY kit from Promega Corporation, Madison WI...
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    • DNA are recovered, but it is degraded, it could be impossible to identify someone. This is why DNA quality is more important than DNA quantity in this project. DNA quality will be measured by counting the number oflod that are amplified, with...
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    • reaction. The 13 core STR loci used in the United States are: CSF1PO, FGA, THO 1 , TPOX, VWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, and D21S11. These thirteen lod have been chosen because of their high discriminatory power,...
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    • 1.5 HYPOTHESIS The purpose of this research is to find methods to successfully extract DNA from duct tape, and to determine if different brands of tape affect the quality of amplified DNA. I hypothesize that at least one method 'Will generate a...
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    • 2.2 EXTRACTION METHOD 1 For this method, a small cutting approximately 5 cm2 was taken from each sample of duct tape and placed into a separate Eppendorf tube. A phenol chloroform organic extraction protocol was then used to remove the DNA from the...
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    • Corporation, Madison, WI [11]. Finally microsatellite analysis was performed using an ABI 3130 genetic analyzer, Applied Biosystems, Foster City, CA, and GeneMapper 4.0 software, Applied Biosystems, Foster City, CA. 2.4 EXTRACTION METHOD 3 For...
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    • 2.9 AIM 2 J\ffiTHODS The three DNA extraction methods previously mentioned were done in order to complete the fIrst goal of this research which was to determine if one of these methods worked better than the others to extract DNA from duct tape...
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    • a KH,JG, TW,Volunteer Initials; RW, Right Wrist; MTH,Mouth; pas, Positive epithelial cell control; HB, Human Blood on Duct tape (positive Control); NEG,Negative control; UNKN, Unknown epithelial cell sample ~T,NoTarget; OC,Overconcentrated; BT,...
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    • "KH, ]G, TW,Volunteer Initials; RW, Right Wrist; MTH,Mouth; pas, Positive epithelial cell control; HB, Human Blood on Duct tape (positive Control); NEG,Negative control; lJNKN, Unknown epithelial cell sample ~T,NoTarget; OC,Overconcentrated; BT,...
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    • CHAPTER 4: DISCUSSION & CONCLUSIONS 4.1 QUANTIFICATION & CONCENTRATION This project was conducted to see how plausible it was to perform DNA analysis on duct tape samples submitted as evidence. The process started with DNA collection and...
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    • 4.2 AMPLIFICATION/STR ANALYSIS Looking at just the quantification data there were quite a few samples that had DNA present, however based on the fragment analysis data, this experiment would indicate that DNA analysis of duct tape is not a very...
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    • damaged or degraded DNA. Also in aim 2, some of the tape adhesives were stronger than the others, specifically Gorilla tape. All four samples from aim 2 that were in range came from Gorilla tape samples. Tbis might indicate that this adhesive was...
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    • REFERENCES 1. Hobbs, Andrea L; Gauntt, Jennifer; Keagy, Roger; Lowe, Preston C; Ward, Dennis. (2007) A New Approach for the Analysis ofDuct Tape Backings: Forensic Science Communications, 9(1). 2. Berg, Jim; Nyberg, Tim. Duct Tape 101:...

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